非水相酶促不可逆转酯反应合成阿魏酸三油酸甘油酯

Enzymatic synthesis of feruloyl-oleylglycerol through irreversible transesterification in nonaqueous phase

摘要:

研究了在有机相甲苯体系下通过阿魏酸乙烯酯（VF）和三油酸甘油酯（TO）的酶促转酯反应合成新型油脂抗氧化剂阿魏酸三油酸甘油酯。使用阿魏酸乙烯酯作为底物可使转酯反应不可逆，这有利于产物产率的提高并缩短反应时间。通过核磁共振和质谱对阿魏酸乙烯酯的结构进行了表征，并讨论了底物摩尔比、反应时间、反应温度、酶用量、水活度对产物产率的影响。结果表明，在以甲苯为溶剂的反应体系下，当底物比n(VF)∶n(TO)=1∶3，反应时间为62 h，反应温度为55℃、酶用量为20 mg/mL，水活度（aw）为0.07时，产物的产率最大，达到96.73%。

Abstract:

The synthesis of a new-type antioxidant of feruloyl-oleylglycerol through lipase-catalyzed irreversible transesterification of vinyl ferulate (VF) and triolein (TO) was studied. Vinyl ferulate made the transesterification reaction irreversible. The structure of VF was identified by NMR and MS and the influential factors of substrate molar ratio, reaction time, reaction temperature, enzyme amount and water activity were systematically studied. Results indicated that in the toluene reaction system the optimal conditions are as follows: n(VF)∶n(TO)=1∶3, reaction time: 62 h, reaction temperature: 55℃, enzyme amount: 20 mg/mL and water activity(aw): 0.07. Under the optimal conditions, the maximum yield as high as 96.73% was obtained.