人工栽培肉苁蓉保肝和抗氧化活性研究

Hepatoprotective effect and antioxidant activity of Cultivate Cistanche deserticola Y. C. Ma

摘要:

采用清除二苯代苦味酰基（DPPH）自由基、清除 [ 2,2'-连氨-（3-乙基苯并噻唑啉-6-磺酸）二氨盐 ] (ABTS) 自由基和铁离子还原/抗氧化能力 (FRAP)测定法评价栽培肉苁蓉不同溶剂提取物体外抗氧化能力；腹腔注射四氯化碳复制小鼠急性肝损伤模型，测定血清谷丙转氨酶 (ALT)、 谷草转氨酶 (AST)活性、肝脏指数和肝组织匀浆中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活力值评价栽培肉苁蓉乙醇提取物的保肝作用。栽培肉苁蓉各提取物均显示较好的清除DPPH自由基、ABTS自由基及还原TPTZ的能力，其中乙醇提取物具有最好的抗氧化活性(三种方法的RACT50值为3534.32、2726.39、2621.68 μmol/g)，与阳性对照药BHA相差不大(RACT50值为4242.31、5457.65、6633.04 μmol/g)，大于BHT (RACT50值为724.59、1322.27、1581.68 μmol/g)；且肝损伤实验显示，乙醇提取物能显著降低小鼠血清中 ALT、 AST活性以及肝匀浆 MDA含量，并能显著提高小鼠肝脏中的SOD值。结果表明，肉苁蓉对四氯化碳致小鼠急性肝损伤具有显著的保护作用，其机制可能与其抗氧化作用有关。

Abstract:

The antioxidant activity of the extracts of Cultivate Cistanche deserticola was assayed by the method of DPPH, ABTS and FRAP in vitro. Mouse model of acute hepatic injury was established by giving CCl 4 abdominally. The activities of ALT and AST in serum and the levels of MDA and SOD in liver tissue were examined to evaluate the hepatoprotective and antioxidant activity of ethanol extract of Cultivate C. deserticola. The ethanol extract was the highest antioxidant activity in four extracts which the values of RACT50 were 3534.32 祄ol/g, 2726.39 祄ol/g and 2621.68 祄ol/g in DPPH, ABTS and FRAP assay respectively. It was slightly lower than that of BHA as positive control, and higher than that of BHT. The ethanol extract of Cultivate C. deserticola significantly reduced the activities of ALT and AST in serum and the levels of MDA in liver tissue, and significantly raised the levels of SOD in liver tissue. The results showed that the ethanol extract of Cultivate C. deserticola had a significant hepatoprotective effect in CCl4-induce acute liver injury. The mechanisms may be related to its effects of reducing lipid peroxidation product.