山胡椒抑制体内外α-糖苷酶活性研究

α-Glucosidase inhibitory activity in vivo and vitro of Lindera glauca (Sieb1et Zucc) Blume

摘要:

本文对山胡椒抑制酵母α-葡萄糖苷酶和大鼠小肠α-葡萄糖苷酶活性进行了研究。利用96微孔板法检测其α-葡萄糖苷酶抑制活性。结果表明，抑制酵母α-葡萄糖苷酶实验中， 山胡椒石油醚部位(IC50=229.70 μg/mL)、乙酸乙酯部位(IC50=259.10 μg/mL)和正丁醇部位(IC50=165.80 μg/mL)活性低于阳性对照Acarbose (IC50=1081.27 μg/mL)；抑制大鼠小肠α-葡萄糖苷酶实验中，仅有乙酸乙酯部位(IC50=418.17 μg/mL)具有活性，阳性对照Acarbose 未检测出其IC50。实验证明，山胡椒各提取部位具有较好抑制酵母α-葡萄糖苷酶活性，但只有乙酸乙酯部位具有良好的大鼠小肠α-葡萄糖苷酶抑制活性。

Abstract:

To investigate the yeast and rat intestinal α-glucosidase inhibitory activity of Lindera glauca (Sieb1et Zucc) Blume. in vitro and vivo. Its α-glucosidase inhibitory activity was assayed by the method of 96-microplates. The results showed that yeast α-glucosidase inhibitory activity of petroleum ether extracts (IC50 = 229.70 μg/mL), ethyl acetate extracts (IC50 =259.10 μg/mL) and n-butanol extracts was lower than that of Acarbose (IC50=1081.27 μg/mL) as positive control, and only ethyl acetate extracts (IC50 =418.17 μg/mL) showed rat intestinal α-glucosidase inhibitory activity. The IC50 value of Acarbose did not be detected. The results indicate that all the extracts of L. glauca showed a better yeast α-glucosidase inhibitory activity and only ethyl acetate extracts showed a better rat intestinal α-glucosidase inhibitory activity.