超声波条件下酶法提取鳝鱼皮中胶原蛋白的工艺研究

Study on the extraction of Collagen with enzyme under supersonic from the skin of Monopterus albus

摘要:

对鳝鱼皮中胶原蛋白的最佳提取工艺条件进行了研究。实验以超声提取时间、胰蛋白酶加入量、酶解时间、提取温度、pH五个因素对提取工艺条件进行了优化。结果表明最佳提取工艺条件为: 酶加入量150U/g、pH7.2、温度35℃、超声波辅助提取时间3min、酶解时间7h，胶原蛋白的提取率达92.4%。利用紫外光谱和傅立叶红外光谱对胶原蛋白进行分析，结果为：胶原蛋白的紫外特征吸收波长为226nm，结构中含有肽键，羰基，羧基等蛋白质的特征官能团。

Abstract:

The extraction conditions of the collagen in the skin of Monopterus albus are studied in this paper. The extraction conditions are optimized by experiment to five factors of extraction time of supersonic,the addition of trypsin, temperature, pH and the decomposition time of enzyme. The result shows that the optimum conditions are supersonic extraction time 3min, the amount of 150U/g ftrypsin, pH7.2, temperature of 35℃, the decomposition time of enzyme 7 hours ,and the extraction rate of collagen is 92.4%. Collagen are analyzed by UV and IR. The result shows that the λmax of product is 226 nm and the functional groups of collagen have peptide bond, carbonyl, carboxyl etc.