木醋杆菌生物合成CMC改性细菌纤维素

Biobynthesis of CMC-Bacterial Cellulose with Acetobacter xylinum

摘要:

以椰子水为培养基，添加羧甲基纤维素(Carboxymethyl cellulose, CMC)可制备羧甲基纤维素改性细菌纤维素(CMC-BC)。CMC可促进产物产量：当CMC添加量为0.6% (w/v%)时，产量达到最大（11.41mg/ml），是纯BC产量(4.73 mg/ml)的2.2倍。采用红外（FT-IR）表征了产物结构，1598cm-1处的吸收峰说明CMC进入了BC中；通过SEM、XRD、TGA研究了产物的微观形态及其性能；并测试了产物的特性粘度与羧甲基化程度。结果显示利用椰子水所制备的CMC-BC缩短了培养时间，增大了产量；并且在CMC添加量较少(0.2-1.8%)时，可改善CMC-BC的性能。

Abstract:

Using coconut-water as main culture medium, carboxymethyl cellulose -bacterial cellulose(CMC-BC) materials were obtained by adding carboxymethyl cellulose (CMC) into the Acetobacter xylinum culture system. The results suggested that CMC-BC polysaccharide produced with coco-nut water had shorter fermentation period and higher yield. And the added CMC can increase the productivity. When 0.6% (w/v%) CMC was added, the yield of dried product film was 10.41 mg/ml compared to 4.73 mg/ml in the control. Their FT-IR spectra suggested that CMC were incorporated into the BC in which the absorption of a carbonyl group at around 1598cm-1 appeared while no appearance in that of BC. Their properties were determined by X-ray diffraction (XRD), scaning electron microscopy(SEM) and thermogravimetric analysis(TGA). The CMC-BCs have the superior properties when the concentration of added CMC were ranging from 0.2% to 1.8%.