酸酶结合法提取牛皮胶原蛋白的工艺研究

Study on extraction of collagen by enzyme-acid method from the cattleskin

摘要:

以新鲜牛皮为原料，分析了牛皮的基本组成；采用胃蛋白酶提取牛皮胶原蛋白，研究了提取过程中加酶量、提取温度、提取时间和料液比对胶原蛋白提取率的影响，得到酸酶结合法提取牛皮中胶原蛋白的最佳工艺条件为：以0.5 mol／L乙酸作为提取剂，料液比为1：30，胃蛋白酶加入量3 000 U／g牛皮，提取温度35℃，提取时间为8 h，在此条件下得胶原蛋白的提取率为61.7％。提取的胶原蛋白经过初步纯化后进行紫外扫描，测得其最大紫外吸收波长为232nm，与sigma标准Ⅰ型胶原蛋白一致。

Abstract:

The collagen from cattle skin has been extracted by using the pepsin, the composition of cattle skin was also analyzed. Effects of enzyme addition, temperature and extract time as well as solid-liquor ratio on extraction efficiency of collagen were investigated in this paper．The optimal processing conditions were obtained by orthogonal experiments as follows: 0.5 mol／L acetic acid as extraction solvent, the added pepsin dosage of 3000 U/g and the ratio of material to liquor 1：30, extraction at 35℃ for 8 h, the recovery yield of the collagens was 61.7%. The maximum absorb wavelength of sample is 232nm by UV scan, which is consistent with the Sigma product.