亳菊香料超声波提取工艺优化及挥发性成分分析

Optimization of Ultrasonic extraction conditions of Chrysanthemum morifolium Ramat flavor concret and the analysis of volatile composition

摘要:

以亳菊香料浸膏提取率为考察指标，采用L9（34）正交试验法进行优选。采用同时蒸馏萃取装置收集挥发性成分并用气相色谱－质谱仪进行分析和鉴定。最佳提取条件为超声波功率800W，提取时间30min，乙醇体积分数70%。共鉴定出88种化合物，用峰面积归一化法测定各组分的百分比为：顺-香橙烯（9.38%）、n-棕榈酸（5.11%）、β-人参烯（4.63%）、长叶醛（4.36%）、a-雪松烯氧化物（3.97%）、桉双烯酮（3.67%）、异香橙烯环氧化物（3.35%）、1-氟-2-[(Z)-2-甲氧基]苯（3.17%）、α-衣兰烯（3.15%）、亚油酸（2.68%）、异香橙烯（2.54%）、α-香附酮（2.35%）等。优选得到的工艺可提高亳菊香料浸膏的提取率。

Abstract:

Taking the extraction efficiency of Chrysanthemum morifolium Ramat flavor concret as the index，the L9（34） orthogonal experimentwas applied to optimize．The volatile compositions in the perfume were prepared with simultaneous distillation and extraction equipment (SDE)．They were isolated and indentified by capillary GC-MS method．The optimal extraction conditmns were obtained as followed：φ (alcohol) = 70％，ultrasonic power 800W and time 30 min．88 peaks compounds were isolated and identified. The relative contents of the total peak area were determined by area normalization method. The main volatile compositions were: (+)-Aromadendrene（9.38%）、n-Hexadecanoic acid（5.11%）、β- Panasinsene（4.63%）、Longifolenaldehyde（4.36%）、α-Cedrene oxide（3.97%）、Eudesma-4(14),11-diene（3.67%）、Isoaromadendrene epoxide（3.35%）、1-Fluoro-2-[(Z)-2-methoxyethenyl]benzene（3.17%）、α-Ylangene（3.15%）、Linolic acid（2.68%）、Alloaromadendren（2.54%）、α-Cyperone（2.35%），etc．The optimum process increased the extraction efficiency of Chrysanthemum morifolium Ramat flavor concret．