离子交换纤维柱分离纯化辣椒碱类化合物

Separation and Purification of Capsaicinoids by Ion Exchange Column

摘要:

实验考察了ZB-2强碱性阴离子交换纤维柱分离纯化指天椒中的辣椒碱类化合物。通过对离子交换纤维吸附前后扫描电镜图分析，表明吸附上了物质，并由红外光谱图分析证实所吸附的物质为辣椒碱类化合物。考察了上柱液pH、质量浓度、流速、上样量与离子交换纤维吸附量的关系，讨论了解吸条件中乙醇体积分数、流速、用量与离子交换纤维解吸率的关系，确定了离子交换纤维柱分离纯化的最佳工艺参数，得到在此条件下辣椒碱类化合物的纯度由4.5 %提高到92.0 %，同时收率达72.3 %。结果表明离子交换纤维柱能快速分离纯化辣椒碱类化合物。

Abstract:

The ZB-2 alkali anion exchange fiber column separating and purifying capsaicinoids from capsicum was investigated in the experiment. Through analyzing the SEM before and after the absorption of capsaicinoids by ion exchange fiber, its absorptive ability was determined. And the absorbed compound was capsaicinoids through analyzing the infrared spectrum. Then the relation between the pH, the mass concentration, the flow velocity and the absorptive capacity was investigated. Furthermore, the relation between the ethanol volume score, the flow velocity, the ethanol volume and the desorptive speed was discussed. Then the optimum technology condition was obtained. Under the condition, the capsaicinoids content enhanced from 4.5 % to 92.0 %, and the yield reached 72.3 %. The result indicates that the ion exchange fiber can separate and purify capsaicinoids.