柴达木枸杞叶有效成分高压均质提取及纯化研究

Study on high pressure homogenization extraction and purification of the main functional components from medlar leaves of Qaidam Basin

摘要:

考察了高压均质提取柴达木枸杞叶有效成分的最佳工艺及对有效成分进行了纯化。在乙醇浓度、料液比、均质压力及提取时间四个单因素实验的基础上利用正交设计对实验进行优化，得出高压均质提取法的最佳工艺条件为：乙醇浓度80%，料液比1:10，均质压力60MPa，提取时间30min，在此条件下，提取物中芦丁含量为10.53%，总黄酮含量为32.61%。分离纯化工艺为：选用AB-8大孔树脂，水洗脱用量5BV，乙醇洗脱用量4BV，经纯化后的产物中，芦丁含量可达60.85%，总黄酮含量可达90.53%。

Abstract:

In this paper, the main functional components was extracted from medlar leaves of Qaidam Basin by high pressure homogenization method and the extract was purified at the optimum condition. The extraction condition was optimized by orthogonal experiment on four simple factors such as ethanol concentration, solid-liquid ratio, extraction pressure, and extraction time. The optimum condition were as follow: ethanol concentration was 80%, solid-liquid ratio was 1:10, extraction pressure was 60MPa, extraction time was 30min. Under the method, rutin content of the extract was 10.53% and total flavonoids content was 32.61%. The condition of purification process was selected AB-8 macroporous resin and some parameters were decided in purification technology that the volume of water 5 BV, the concentration of used ethanol gradient and the adding volume 4 BV. Rutin content of purifications was up to 60.85% and total flavonoids content was up to 90.53% in these conditions.