超临界CO2抗溶剂法制备白藜芦醇-玉米蛋白纳米粒

Preparation of Resveratrol-loaded Zein Nanoparticles with Supercritical CO2 Anti-solvent Techniques

摘要:

以玉米蛋白为壁材，白藜芦醇为模型药物，采用超临界CO2抗溶剂法制备负载白藜芦醇的玉米蛋白纳米粒，考察了过程参数压力、温度以及芯材比对白藜芦醇在纳米粒中负载量的影响。用紫外-可见光谱分析、扫描电镜、X射线衍射分析等方法对样品进行表征，并且对释放度进行研究。结果表明，随着超临界CO2压力的增加，白藜芦醇的负载量降低；随着温度和芯材比的增加，白藜芦醇的负载量升高。玉米蛋白和白藜芦醇形成Matrix结构，纳米粒为均匀球形颗粒。释放度实验显示，纳米粒中白藜芦醇的释放速度比原料的释放速度慢，有缓释作用。

Abstract:

Supercritical CO2 anti-solvent technology was tested to prepare zein nanoparticles loaded with resveratrol. The effects of different operating parameters, such as pressure, temperature, and ratio on the loading weight of resveratrol were investigated UV, SEM, XRD were used to characterize the products. The release behavior of resveratrol from the prepared nanoparticles was also studied. Results show that the yield of resveratrol is lower when increasing CO2 pressure. However, while temperature and ratio are increased, the loading yield is higher The structure of resveratrol-loaded zein nanoparticle is a Matrix with good sphere shape。Furthermore, in vitro drug release studies show that products exhibit a slower release than the single resveratrol.