微波辅助顶空固相微萃取法快速分析黔产一年蓬不同部位挥发油化学成分

Rapid Analysis of Volatile Oils Chemical Composition in Different Parts of Herba Erigerontis Annui in Guizhou by MAE-HS-SPME Followed by GC–MS

摘要:

采用微波辅助顶空固相微萃取法(MAE-HS-SPME)快速分析一年蓬不同部位的挥发性化学成分，通过气相色谱-质谱法与Kovats色谱保留指数相结合进行定性定量分析，在一年蓬的茎、叶和花中分别都鉴定出67、69和69种化合物，分别占挥发油总峰面积的92.71%、96.72%和96.16%，在茎中主要成分是β-榄香烯(5.22%)，大根香叶烯d (41.80%)和（ ）-β-蛇床烯(7.71%)。叶中主要成分是甲基环戊烯醇酮(6.43%)，β-榄香烯(8.45%)，大根香叶烯 d (40.17%)和 (E,E)-α-金合欢烯(5.80%)。而在花中主要是甲基环戊烯醇酮(4.20%)，β-榄香烯(8.65%)和大根香叶烯d (31.20%)。且相对含量有显著差异。

Abstract:

The volatile compounds were extracted in the different parts of Herba Erigerontis Annui by microwave-assisted headspace solid-phase micro-extraction(MAE-HS-SPME). Their constituents were analyzed and identified by gas chromatography-mass spectrometry combined with Kovats retention index, The results showed that 67,69 and 69 volatile constituents in the stem, in the leaf and in the flower of Herba Erigerontis Annui were separated and identified. Accounting for 92.71%, 96.72% and 96.16% of the total peak areas, respectively. Which is the main component in stem β-Elemene (5.22%), germacrene d (41.80%) and( )-β- selinene (7.71%).And in the leaves cyclotene (6.43%),β-Elemene (8.45%), germacrene d (40.17%) and (E,E)-α- Farnesene(5.80%).And in the Flowers cyclotene (4.20%), β-Elemene (8.65%) and germacrene d (31.20%).It was also observed that there were significant differences in the relative contents of three parts.