响应曲面法优化超声辅助提取油菜籽皮中的原花青素

Optimization of ultrasound-assisted extraction of proanthocyanidin from rapeseed hull using response surface methodology

摘要:

以乙醇为提取剂，用超声辅助的方法从油菜籽皮中提取了原花青素。在讨论了提取剂体积分数、提取时间、液料比和超声波功率等单因素试验的基础上，运用Box-Behnken中心组合试验和响应曲面法分析了提取剂体积分数、提取时间和液料比3个因素对原花青素提取率的影响，并优化了提取工艺。结果表明，最佳的工艺条件为：乙醇体积分数62.9%，提取时间20.8min，液料比25mL/g。在此条件下，原花青素提取率的预测值为5.05mg/g，验证实验值为5.02mg/g，说明响应曲面法优化油菜籽皮中原花青素提取工艺是可行的。

Abstract:

Ultrasound assisted extraction of proanthocyanidin from rapeseed hull using ethanol as the extraction solvent was studied. Based on the single factor tests of ethanol volume fraction, extraction time, liquid-solid ratio and ultrasound power, the optimum process were evaluated by Box-Behnken central composite experimental design and response surface methodology analysis. The results indicated that ethanol volume fraction 62.9%, extraction time of 20.8min and liquid-solid ratio of 25mL/g were found optimum. Under these optimized conditions, the experimental value of proanthocyanidin extraction yield 5.05mg/g, in good agreement with the predictive value 5.02mg/g. Therefore, the ultrasound-assisted extraction of proanthocyanidin from rapeseed hull using response surface methodology is very reliable.