中草药还原法制备银纳米颗粒及其抗菌性能表征

Biosynthesis of silver nanoparticles using various medicinal plant extracts and their antibacterial properties

摘要:

分别以中草药丁香、山茱萸、地榆和乌梅的提取液合成银纳米颗粒，利用紫外-可见吸收光谱（UV-Vis）、透射电镜（TEM）以及X射线粉末衍射（XRD）对产物进行了表征。结果表明，所得银纳米颗粒呈近球形，提高提取液的pH值有利于获得粒径较小、分散性好、稳定性高的银纳米颗粒。进一步考察制得的银纳米颗粒的抗菌性能，结果表明其对大肠杆菌和金黄色葡萄球菌有很强的抑制作用，最小抑菌浓度（MIC）分别可达1.69和3.38 μg/mL。

Abstract:

Quasi-spherical silver nanoparticles (AgNPs) were synthesized by the extracts of different medicinal plants including Syzygium aromaticum, Cornus officinalis, Prunus mume and Sanguisorba officinalis, and were characterized by UV-Visible spectroscopy, transmission electron microscopy and X-ray diffraction. When pH values of the extracts were adjusted to 10, the biosynthesized AgNPs were well-dispersed and highly stable, and are relatively small in size. Thus obtained AgNPs were found to demonstrate good antibacterial activity against Escherichia coli and Staphylococcus aureus, with minimum inhibition concentrations of 1.69 and 3.38 μg/mL required, respectively.