响应面法优化水/醇处理后汽爆玉米秸秆酶解的研究

tudy on Optimization of Enzymatic Hydrolysis Process of Corn Stalk by Water/ Ethanol Extraction after Steam Explosion

摘要:

为了提高水/醇处理后汽爆玉米秸秆的酶解还原糖产率，对其酶解条件进行了优化。通过响应面优化法确定了底物浓度为53.28g/L，纤维素酶用量为53.32FPU/g，酶解时间为60.45h时，还原糖产率可达672.36mg/g，与秸秆物料及汽爆后物料相比，酶解还原糖产率分别提高了179.21%和37.29%。化学组分及结构形貌分析表明：水/醇处理后物料纤维素含量显著增加，X射线衍射(XRD)和扫描电镜(SEM)结果表明经过水/醇处理后物料相对结晶度增高，但结构更有利于纤维素酶分子的吸附。

Abstract:

In order to improve the production of reducing sugar yield from corn stalk by water/ ethanol extraction after steam explosion, the enzymatic hydrolysis process was optimized. With the response surface methodology, the production of reducing sugar yield can reach 672.36mg/g, when the substrate concentration of 53.8g/L, cellulase loading of 53.32FPU/g and reaction time of 60.45h. Compare to untreated and steam exploded corn stalks, the production of reducing sugar yield increased by 179.21% and 37.29%. Chemical compositions of untreated/treated corn stalk were investigated and their structures were also detected by Scanning Electron Microscope (SEM) and X-ray diffraction (XRD). The results showed that: the relative content of cellulose in corn stalk treated by water/ ethanol extraction increased significantly, the relative crystallinity increased, but the changes of structure lead to the cellulase attach on easier.