不同方法提取鸦胆子挥发油化学成分的GC-MS分析

Analysis of the Essential Oils from Brucea javanica with Different Extraction Methods by Gas Chromatography-Mass Spectrometry

摘要:

采用水蒸气蒸馏法和微波辅助提取鸦胆子挥发油。运用毛细管气相色谱-质谱联用仪结合计算机检索对所得挥发油成分进行分析和鉴定，用气相色谱面积归一法测定各组分的相对百分含量。结果显示三种方法分别从鸦胆子所得挥发油中鉴定出42及37种化合物，用气相色谱峰面积归一法测定各组分的相对质量分数，各占总峰面积的94.09%和94.13%。不同方法所得鸦胆子挥发油化学组成各有异同，但主要成分是黄樟脑、( )-4-蒈烯、?-香叶烯、丁香油酚甲醚、2,6-二甲氧基甲苯、柠檬烯和甲基胡椒酚。

Abstract:

Brucea javanica (Simaroubaceae) is a medicinal plant traditionally used in Southern of China to treat several diseases. In order to enlarge the medication parts, the constituents of essential oils which extracted with microwave-assisted hydrodistillation (MAHD) and hydrodistillation (HD) from Brucea javanica were analyzed by capillary GC-MS and elucidated based on the standard mass spectral data. 42 and 37 kinds of volatile components were identified respectively. Relative mass fractions of the constituents were determined by area normalization method. The identified relative contents of essential oils were 94.09% and 94.13%, respectively. There were differences among some chemical constituents of two kinds of essential oils. But their main constituents were safrole, ( )-4-carene, ?-myrcene, eugenol methyl ether, 2,6-dimethoxy toluene, limonene and estragole.