药对厚朴-麻黄及其单味药中挥发油成分GC-MS的比较分析

Comparative analysis of volatile constituents in herbal pair Cortex Magnoliae Officinalis -Herba Ephedrae and its signal herb by GC-MS

摘要:

本文采用水蒸气蒸馏分别对药对厚朴-麻黄及单味药麻黄和厚朴的挥发油成进行了提取，并结合GC-MS对所得样品化学成分进行了分离和结构鉴定，用气相色谱面积归一法测定各组分的相对百分含量。结果显示麻黄-厚朴、麻黄和厚朴挥发油分别鉴定了44，38和39个色谱峰，分别占挥发油总峰面积的98.42%，99.07%和99.21%。药对麻黄-厚朴中挥发油化学成分有28 种来自厚朴，25 种来自麻黄，新产生了6 种化合物。

Abstract:

In this paper, the constituents of essential oils which extracted with hydrodistillation from the herbal pair Cortex Magnoliae Officinalis -Herba Ephedrae (CMO-HE) and its signal herbs were analyzed by capillary GC-MS and elucidated based on the standard mass spectral data. 44, 38 and 39 kinds of volatile components were identified respectively. Relative mass fractions of the constituents were determined by area normalization method. The identified relative contents of essential oils were 98.42%, 99.07% and 99.21%, respectively. It is further demonstrated that 28 and 25 volatile constituents of CMO-HE are from that of single herb CMO and HE, respectively. Ten kinds of new volatile compounds in CMO-HE are produced because of the chemical reactions and physical changes occurred in the process of decocting two single herbs.