离子液体[BMIM]Cl处理汽爆活化玉米秸秆及酶解

Enzymatic hydrolysis of corn stalk treated by ionic liquid [BMIM]Cl after steam explosion activation

摘要:

为了提高玉米秸秆酶解还原糖产率，利用蒸汽爆破法活化玉米秸秆原料，并利用离子液体[BMIM]Cl进行处理，考察了汽爆压力和维压时间对处理后物料酶解还原糖产率的影响。结果表明：汽爆压力2.6MPa，维压时间90s下汽爆活化秸秆原料，[BMIM]Cl处理后，酶解24h后还原糖产率较汽爆活化后物料提高了84.03%，较原料提高了286.83%。秸秆化学组分分析表明，[BMIM]Cl处理后物料纤维素质量分数增加了64.86%，X射线衍射(XRD)与扫描电镜(SEM)分析表明，其晶型结构转变为无定形结构，更有利于纤维素酶与底物作用。说明汽爆活化[BMIM]Cl处理能显著提高玉米秸秆的酶解还原糖产率。

Abstract:

For improving the yield of reducing sugars from corn stalk enzymatic hydrolysis，the steam explosion was selected as an activation method，ionic liquid [BMIM]Cl was used as a further treatment step, the influence of steam explosion pressure and time on the the yield of reducing sugars was evaluated. The results showed that after activating with a steam pressure of 2.6 MPa for 90 seconds treatment, the yield of reducing sugars from [BMIM]Cl treated corn stalk can increase by 84.03% compare to activated corn stalk and by 286.83% compare to untreated corn stalk. The relative content of cellulose in [BMIM]Cl treated corn stalk increased by 64.86%. X-ray diffraction analysis (XRD) and Scanning electronic microscopy (SEM) indicated that the structure of [BMIM]Cl treated corn stalk changed into amorphous. The changes of structure lead to the cellulase attach on easier. So it came to the conclusion that corn stalk treated by [BMIM]Cl and activated by steam explosion can enhancing the yield of reducing sugars significantly.