The effecting factors of degumming and deacidification for crude Jatropha oil were investigated with the activated clay loaded by the citric acid and the calcium hydroxide respectively. And then degumming and deacidification process were coupled together to strengthen refine process by one-step. The results indicated that the acid value is 0.23 mg KOH/g and the phosphatide content is 0.09 mg/g for refined Jatropha oil. When the refined oil was the feedstock using a homogeneous alkaline catalyst for biodiesel production, the content of methyl ester in the product is larger than 99%. The performance on biodiesel production has been satisfactory.