海南核果木抗氧化活性研究

Antioxidant Activity of Extracts from Drypetes Hainanensis Merr.

摘要:

将海南核果木干燥枝叶用乙醇浸提，对总提物分别用石油醚、乙酸乙酯、正丁醇萃取，并以DPPH法和FRAP法对总提物及各萃取物的抗氧化活性进行评价。结果显示，在DPPH法中海南核果木总提物（DH）、石油醚萃取物（DHMSO）、乙酸乙酯萃取物（DHEtOAC）、正丁醇萃取物（DHNBA）、水相（DHH2O）的IC50分别为77.3、115.2、51.4、342.9、205.7、10.7μg/mL，其FRAP值分别为11712.2±931.8、10820.9±690.5、14386.4±568.8、1157.6±63.3、5584.1±182.3μmol Fe2+/g。且抗坏血酸（Vc）和BHT的IC50分别为10.7、47.1 μg/mL，FRAP值分别为18022.8±63.3、14568.7±137.0 μmol Fe2+/g，并通过多元聚类分析表明DH、DHMSO、DHEtOAC与BHT、Vc具有类似强度的抗氧化活性，可归为同一类型，且DHEtOAC抗氧化活性与BHT无显著性差异，说明其粗提取具有较强的抗氧化活性，在医药、保健、化妆品等行业具有广阔的研究与应用前景。

Abstract:

The dry stems and leaves of Drypetes hainanensis were extracted with ethnol, then the total extracts were respectively extracted with petroleum ether, ethyl acetate and n-butanol. Whereafter, the antioxidant activities of the total extracts with ethnol and all the other extractions were investigated with DPPH and FRAP assay. The results displayed that the IC50 of extracts of D.hainanensis (DH), extracts of petroleum ether (DHMSO), extracts of ethyl acetate (DHEtOAC), extracts of n-butanol (DHNBA), extracts of water (DHH2O) were 77.3, 115.2, 51.4, 342.9, and 205.7 μg/mL, respectively, with DPPH method, and their FRAP values were 11712.2±931.8, 10820.9±690.5, 14386.4±568.8, 1157.6±63.3, 5584.1±182.3 μmol Fe2+/g, respectively, as well. The IC50 of ascorbic acid (Vc) and BHT are respectively 10.7 and 47.1 μg/mL, and their FRAP values are 18022.8±63.3 and 14568.7±137.0 μmol Fe2+/g, respectively. In addition, multi-cluster showed that DH, DHMSO, DHEtOAC, BHT and Vc had a similar intensity of antioxidant activity, therefore, they could be classified into the same type material. Also, it was found that the antioxidant activity of DHEtOAC could be comparable with BHT, accordingly, the crude extracts had strong antioxidant activity. So in those domains of pharmaceutical, health care, cosmetics and so on forth, D.hainanensis has wide-ranging research and application prospects.