酸碱法纯化红三叶草中总异黄酮的工艺研究

Study on Acid-base Method Purification of Isoflavones in Red Clover

摘要:

采用酸碱法及醇溶水沉法对红三叶草中总异黄酮进行了纯化工艺的研究，分别考察碱溶pH、酸沉pH、水沉加水量对异黄酮含量及浸膏得率的影响。再通过正交实验得到最佳纯化工艺为：碱溶的pH为8.5，酸沉的pH为2.5时，加水量为6倍量时，异黄酮含量可达50%以上。

Abstract:

The purification technology of the total isoflavones from red clover was studied with a method of acid-base and water-precipitation.In the paper,the effect of alkaline solution,acid settling and the amount of water were investigated.By the orthogonal test L9(33),the optimal purification conditions are as follows:pH 8.5 of alkaline solution,pH 2.5 of Acid settling，six times of the amount of water.Under the best conditions the content of the total isoflavones from red clover can reach 50％．