溶剂脱毒汽爆玉米秸秆对酶解以及发酵的影响

Impact of Detoxification of Steam Exploded Corn Straw on Enzymatic Hydrolysis and Ethanol Fermentation by Different Organic Solvents

摘要:

以脱毒对酶解以及发酵的影响为研究对象，以酶解还原糖得率以及发酵乙醇浓度为指标，采用溶剂萃取的方法对无催化汽爆玉米秸秆进行萃取脱毒。结果表明，酶解还原糖得率随着萃取剂以及萃取方式介于34.85和89.7%，酶解还原糖得率和发酵乙醇浓度与脱毒有机溶剂的的沸点高度负相关，表明有机溶剂的残留是导致酶失活的主要原因。而对于所考察的溶剂，乙醇产率介于0.47-0.49g乙醇/g还原糖，表明有机溶剂残留对乙醇发酵并无显著影响。采用乙醚和丙酮的组合萃取，乙醇最高产率可以达到理论值的96.1%。

Abstract:

Reducing sugar yield and ethanol concentration was used as the indexes to probe the effect of detoxification on enzymatic hydrolysis and ethanol fermentation by using different organic solvents to extract the inhibitors from the catalyst free steam exploded corn straw as detoxification method. The reducing sugar yield varied from 34.8 to 89.7% depending on the detoxification solvents and modes. The results showed that both reducing sugar yield and ethanol concentration showed highly negative correlation to the boiling points of the organic solvents, which indicate that the solvent residue is the main reason of enzyme inactivation. However, the ethanol yield varied from 0.47 to 0.49g ethanol / g reducing sugar for the studied extractive organic solvents,，which shows that solvent residue had no significant effect on the ethanol fermentation. The highest ethanol yield (96.1% of theoretical value) was obtained with the combined extraction of ether and acetone.