左氧氟沙星对铜绿假单胞菌NY3产表面活性剂特性的影响作用

Effects of Levofloxacin on the production and the structure of rhamnolipids produced by pseudomonas aeruginosa NY3

摘要:

利用左氧氟沙星诱变铜绿假单胞菌NY3获得NY3-1和NY3-2诱变株，通过比较所产表面活性剂的理化性能可知：诱变株和野生株所产鼠李糖脂表面活性剂的产量、成分和结构特征有明显的差异。与野生菌株相比，NY3-1产量提高19.57%，NY3-2产量降低17.60%；NY3、NY3-1、NY3-2所产鼠李糖脂的双/单糖含量比例分别为0.969、0.677、1.316，红外光谱图显示，NY3所产鼠李糖脂中不含C=C键，而经左氧氟沙星诱导后的NY3-1和NY3-2分泌的鼠李糖脂中均含有C=C不饱和键。比较ESI-MS质谱发现，三种菌株所产鼠李糖脂中都含有Rh-C10，诱变菌株所产鼠李糖脂中含有不饱和键的Rha-Rha-C10:1，且含量较高。

Abstract:

Two mutagenesis strains named NY3-1 and NY3-2 were obtained through pseudomonas aeruginosa NY3 inducing with levofloxacin. By comparing the physical and chemical properties of rhamnolipids produced by them, some conclusions were reached as follows: Production, composition and structure characteristics of rhamnolipids produced by mutagenesis strains and wild strains were quite different. Compared with the wild strains，yield of rhamnolipids produced by NY3-1 was improved by 19.57%, while for NY3-2 the yield was decreased by 17.60%. the ratios of amounts of disaccharide and monosaccharide in rhamnolipids produced by NY3、NY3-1、NY3-2 strains were 0.969,0.677 and 1.316 respectively. The infra-red spectrums demonstrated that no C=C unsaturated bond was found in the rhamnolipids produced by NY3 strains, while those produced by NY3-1 and NY3-2 strains contained C=C bond. The results from electrospray ionization mass spectrometry (ESI-MS) analysis showed that all the rhannolipids produced by the three stains contained Rh-C10,while only that produced by two induced strains have high content of Rha-Rha-C10:1 which contain C=C unsaturated bond.