固定化沙雷氏菌脂肪酶拆分(R,S)-2-羟基-4-苯基丁酸乙酯

Resolution of (R,S)-2-hydroxy-4-Ethyl benzene by Immobilized Serratia sp. Lipase

摘要:

利用海藻酸钙-戊二醛交联法对本实验室筛选到的一株能选择性拆分(R,S)-2-羟基-4-苯基丁酸乙酯[(R,S)-HPBE]的沙雷氏菌脂肪酶进行固定化，并对固定化脂肪酶的酶活和拆分条件进行了研究。结果表明: 其最适反应温度为50 ℃，pH=7.0, 底物浓度40 mmol/L，ρ(酶液)=200 g/L, 在该条件下,反应10 h后，(R)-2-羟基-4-苯基丁酸乙酯[(R)-HPBE]产率达93.4%,光学纯度（e.e.）为96.2%。连续反应12批次，固定化脂肪酶仍可以使(R)-HPBE产率维持在80%以上。

Abstract:

Optical resolution of (R,S)-2-hydroxy-4-Ethyl benzene [(R,S)-HPBE] by calcium alginate-glutaraldehyde cross-linking Serratia sp. lipase was developed, and the activity and many factors affecting the resolution were investigated. The optimum reaction conditions were temperature 50℃, pH=7.0, concentration of (R,S)-HPBE 40 mmol/L and ρ(lipase amount)=200 g/L. After 10 h of resolution by immobilized lipase, the conversion rate of (R)-HPBE is 93.4% and e.e. is 96.2%. After twelve times repeated batch operation, the conversion rate of product by immobilized lipase is more than 80%.