荆条蜂胶化学成分的研究

Studies on Chemical Constituents of Jingtiao Propolis

摘要:

本文对荆条蜂胶乙醇提取物的化学成分进行了研究，为进一步的活性评价及建立标准化模型提供基础数据。蜂胶原胶经65%乙醇溶液80℃加热得到蜂胶提取物，将提取物采用石油醚、乙酸乙酯、正丁醇进行逐级溶剂分配、硅胶色谱柱层析、重结晶、制备液相等方法进行分离和纯化，从乙酸乙酯部分分离得到31个化合物，通过对化学物理性质分析以及红外、紫外、质谱、核磁共振波谱的测定解析，确定了化合物结构。其中黄酮类化合物14个，肉桂酸衍生物7个，咖啡酸酯类化合物6个，其它类4个。

Abstract:

This paper was carried out to investigate the chemical compositions and their quantitive analytical methods of Jingtiao propolis collected from Liaoning province for the first time. Propolis sample is extracted with 65% ethanol at 80℃，the extract is subjected to concentration under reduced pressure，solvent partition，silica gel column chromatography，recrystallization and high preparative performance liquid chromatography. Thirty one compounds are obtained and their structures are elucidated on the basis of the data of IR，UV，MS，1H-NMR，13C-NMR and physical properties. Fourteen of which are flavanoids，seven are caffeic acid analogues，six are caffeic esters，and four are other categories.