夏枯草酸性多糖的理化性质分析及其抗氧化活性
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TQ 207,1

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Physical-Chemical Properties Analysis and Antioxidant Activity of Acidic Polysaccharide from Prunella vulgaris Linn
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    摘要:

    水提醇沉后的夏枯草粗多糖用HZ-820大孔吸附树脂脱色素和蛋白质,再经DEAE-Sepharose Fast Flow离子交换柱色谱和Sephadex G-50凝胶柱色谱进一步纯化得到高纯度酸性多糖,用气相色谱、高效凝胶渗透色谱、红外、热稳定性、扫描电镜等理化性质分析,最后再进行其抗氧化活性研究。结果表明:夏枯草酸性多糖中含有鼠李糖、木糖、阿拉伯糖、葡萄糖和甘露糖,其含量分别为0.9857%、1.7568%、3.2029%、1.9753%、0.6806%,重均分子量为57234Da。夏枯草酸性多糖在230℃开始发生降解,600℃基本结束,其具有较好的热稳定性。夏枯草酸性多糖表面基本光滑,呈薄片状且无规则卷曲。抗氧化研究表明,夏枯草酸性多糖能够有效的清除NO2-自由基、同时具有较强的还原能力和对铁离子的螯合能力。

    Abstract:

    Crude polysaccharides obtained from Prunella vulgaris Linn by boiling water extraction and ethanol deposition, was decolorized and deproteinized with HZ-820-type resin adsorptive and further purifed by DEAE-Sepharose Fast Flow ion-exchange column chromatography and Sephadex G-50 Gel column chromatography. After that, the physicochemical properties of purified acidic polysaccharide were analyzed by gas chromatography, infrared, thermal analysis and scanning electron microscopy. Finally, antioxidant activities of it were also studied. The results exhibited that the acidic polysaccharide consisted of rhamnose, xylose, arabinose, glucose and mannose, the content of them were 0.9857%、 1.7568%、3.2029%、1.9753% and 0.6806%, respectively. The weight-average molecular weight of acidic polysaccharide was 57234Da. The acidic polysaccharide began to degrade at 230℃ and the degradation ended at 600℃, which have better thermal stability. Meaning while, it have smooth surface, random coil and present thin slice. The antioxidant activity showed that acidic polysaccharide of Prunella vulgaris Linn had eliminating capability on NO2- radical, and also had significant reducing power and the chelating ability on ferrous ions.

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王莹莹,熊双丽.夏枯草酸性多糖的理化性质分析及其抗氧化活性[J].精细化工,2012,29(5):

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  • 收稿日期:2011-12-07
  • 最后修改日期:2012-02-14
  • 录用日期:2012-02-17
  • 在线发布日期: 2012-04-13
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