生物法高效合成氢过氧化亚油酸乙酯

Efficient Synthesis of Ethyl Linoleic Acid Hydroperoxide

摘要:

该文以亚油酸乙酯为底物，开发了一条利用豆粕为直接催化剂，在线偶联脂氧酶的提取和固定化的高效合成氢过氧化物的方法。对影响氢过氧化亚油酸乙酯产率的各种因素，温度、pH以及催化剂和底物用量等进行了研究。豆粕催化氧化亚油酸乙酯的最佳反应条件为：1.5 g亚油酸乙酯，1.5 g豆粕，0.45 mL乙醇溶于7.5 mL pH6 磷酸盐缓冲液，15 oC下反应2 h，产率达55%。该方法不仅省去提取、纯化和固定化等工序，而且大为提高了LOX反应的催化效率和产率，使该生物转化过程更接近于工业化生产需要。

Abstract:

With simultaneous enzyme releasing and lipoxygenation, the catalytic performance of soymeal to catalyze the hydroperoxidation reaction was evaluated, using ethyl linoleate as the substrate. The influences of various parameters relating to the hydroperoxide yield, including pH, temperature, dosage of soymeal and ethyl linoleate were investigated. The optimal conditions for ethyl linoleate were 1.5 g ethyl linoleate in 7.5 mL phosphate buffer solution of pH 6 with 0.45 mL ethanol as co-solvent reacting for 2 h at 15 oC, and the yield reached 55%, which is much higher than the previously reported(15%). Soymeal has much better catalytic performance than LOX solution. The application of soymeal not only reduces the number of steps by coupling extraction of LOX and oxidation reaction, but also increased the total yield.