显色底物磷脂酰肌醇的条件优化及应用效果研究

Optimization and Application of l-α-phosphatidylinositol as a chromogenic substrate

摘要:

采用化学方法制备单核细胞增生李斯特菌（Listeria monocytogenes，LM）显色培养基底物磷脂酰肌醇（l-α-phosphatidylinositol，PI）。PI提纯过程中纯度和回收率是一对难以同时提高的矛盾因素，实验采用单一及复合溶剂萃取相结合的方法，具体考察了溶剂比、溶剂体积、碱性物质种类等条件对该矛盾因素的影响。经过优化研究，碱性甲醇溶液预处理后，采用V(正己烷)：V（乙醇溶液）=1:1.5、氨水为碱性物质、pH=8.0时分离效果最佳。比较了纯化前后产物与标准品的微生物显色效果，结果证实了产品具有可观的应用价值。

Abstract:

Abstract: Chemical method was adapted to prepare the key substrate , l – α – phosphatidylinositol(PI）, of Listeria monocytogenes(LM） chromogenic medium. The purity and the yield were contradict factors which could not be improved both in the extraction of PI. The experiment combined single and mixed solvent methods to extract PI, especially investigated the influences of solvent ratio, solvent volume, the kind of alkalinity substances to the purity and yield of the products. The optimal conditions appeared after pretreatment of alkali methanol, under the solvent ratio of V(n-hexane) ： V(ethanol solution) = 1:1.5, using aqueous ammonia as alkalinity substance, and pH = 8.0. The comparison of color development among the PI before and after the purification and the product purchased from abroad, showed that the product had a promising application future.