脂质体包裹胶原蛋白的制备及其透皮性能

Preparation of collagenic Liposomes and performance through skin

摘要:

为提高胶原蛋白体外透皮吸收性能，以卵磷脂和胆固醇为成膜材料，薄膜分散法制备了鱼皮水解胶原蛋白脂质体。以模拟Franze扩散池进行小鼠离体皮肤渗透扩散试验，HPLC测定胶原蛋白含量，考察胶原蛋白脂质体的透皮吸收量与吸收率。实验结果表明分子量3500Da的鱼皮水解胶原蛋白经脂质体包封后，透皮吸收量在24h内达到21.9%，较直接涂抹胶原蛋白的透皮吸收率提高一倍。证明小分子胶原蛋白经脂质体包封能够改善其体外透皮吸收性能。

Abstract:

In order to improve the collagen in vitro absorbed through skin performance, prepared fishskin hydrolyzed collagen liposome with lecithin and cholesterol by using film materials method.Using emulational Franze diffusion pool to do diffusion test with excised skin of male mice, detected Collagen protein content by HPLC. Investigated the content of collagen liposome which uptake through skin and absorption rate. The experimental results show that the molecular weight of 3500 Da fishskin hydrolyzed collagen the liposome after sealing bag, 21.9% can through skin within 24h, doubled a direct daub collagen passes the skin absorption rate. It be proved that small molecules collagen encapsulated by liposomes can improve performance absorbed through skin in vitro.