龙眼核中的神经酰胺和脑苷脂的分离和鉴定

Isolation and identification of ceramides and cerebrosides from longan (Dimocarpus longan Lour.) seeds

摘要:

研究龙眼(Dimocarpus longan Lour.)果核的化学成分，采用硅胶柱色谱对龙眼果核石油醚提取物和95%乙醇提取物进行分离，并利用理化性质和光谱数据鉴定化合物的结构。从龙眼果核的提取物中分离得到一组混合神经酰胺（化合物1、2）和一组混合脑苷脂（化合物36），结构鉴定为Rel-(3S,4S,5S)-3-[(2′R)-2′-羟基二十二酰胺]-4-羟基-5-[(4′′Z)-十四烷-4′′-烯]-2,3,4,5-四氢呋喃（1）、Rel-(3S,4S,5S)-3-[(2′R)-2′-羟基二十四酰胺]-4-羟基-5-[(4′′Z)-十四烷-4′′-烯]-2,3,4,5-四氢呋喃（2）、1-O-(β-D-吡喃葡萄糖基)-(2S,3S,4R,8E)-2-[2′-羟基二十四酰胺]-8-十八烯-1,3,4-三醇（3）、1-O-(β-D-吡喃葡萄糖基)-(2S,3S,4R,8Z)-2-[2′-羟基二十四酰胺]-8-十八烯-1,3,4-三醇（4）、1-O-(β-D-吡喃葡萄糖基)-(2S,3S,4R,8E)-2-[2′-羟基二十二酰胺]-8-十八烯-1,3,4-三醇（5）、1-O-(β-D-吡喃葡萄糖基)-(2S,3S,4R,8Z)-2-[2′-羟基二十二酰胺]-8-十八烯-1,3,4-三醇（6）。

Abstract:

To investigate the chemical constituents of longan (Dimocarpus longan Lour.) seeds, a ceramides molecular species (compounds 12) were isolated from petroleum ether extract and a cerebroside molecular species (compounds 36) from the w(ethanol)=95% extract on longan seeds by silica gel column chromatography, Their structures were identified on the basis of physical and chemical properties and spectral analysis. compounds 12 and compounds 36 isolated identified as: Rel-(3S,4S,5S)-3-[(2′R)-2′-hydroxydocosylamino]-4-hydroxy-5-[(4′′Z)-tetradecane -4′′ -ene]-2,3,4,5-tetrahydrofuran(1), Rel-(3S,4S,5S)-3-[(2′R)-2′-hydroxytetracosanoyl- amino]-4-hydroxy-5-[(4′′Z)-tetradecane-4′′-ene]-2,3,4,5-tetrahydrofuran(2), 1-O-β-D- glucopyranosyl-(2S,3S,4R,8E)-2-[2′-hydroxylignoceroylamino]-8-octadecene-1,3,4- triol(3), 1-O-β-D-glucopyranosyl-(2S,3S,4R,8Z)-2-[2′-hydroxylignoceroylamino]-8- octadecene-1,3,4-triol(4), 1-O-β-D-glucopyranosyl-(2S,3S,4R,8E)-2-[2′-hydroxyldoc- osylamino]-8-octadecene-1,3,4-triol(5), 1-O-β-D-glucopyranosyl-(2S,3S,4R,8Z)-2-[2′ - hydroxydocosylamino]-8-octadecene-1,3,4-triol(6).