正交设计优化超临界二氧化碳萃取喜树种子中喜树碱

Optimization of Supercritical CO2 Extraction of Camptothecin from Camptotheca acuminata Seeds Using Orthogonal Experimental Design

摘要:

用超临界CO2流体萃取技术从喜树种子中提取喜树碱。在CO2流量20 L/h的条件下，探讨了夹带剂、萃取压力、萃取温度和萃取时间对喜树种子中喜树碱提取率的影响。通过正交实验设计验证并确定了超临界CO2萃取喜树种子中喜树碱的最佳工艺：萃取压力25 MPa，萃取温度50 ℃，萃取时间120 min，夹带剂乙醇体积分数90 %。在此条件下，喜树碱平均提取率为76.98%，所得到的萃取物中喜树碱的质量分数达43.68%。

Abstract:

Supercritical CO2 fluid extraction technology was used to extract camptothecin from Camptotheca acuminate seeds. With the preconditions of CO2 flux 20 L/h, the effect of different parameters such as extraction time, temperature, extraction pressure and entrainer on the supercritical carbon dioxide extraction of camptothecin was investigated. The optimum technological conditions were validated by orthogonal experiments design: extraction press 25MPa, temperature 50℃, extraction time 120 min, and entrainer 90% ethanol. Under the optimal conditions, extraction rates of camptothecin was 76.98%, content of camptothecin was 43.68% in crude extracts.