发酵-渗透汽化连续耦合生产ABE的动力学研究

Study on kinetics of ABE production by continuously coupling the fermentation and pervaporation

摘要:

采用PDMS膜生物反应器和丙酮丁醇梭菌进行了生产ABE的封闭循环连续发酵实验，研究了发酵和渗透汽化分离连续耦合条件下的发酵动力学行为。发酵-分离连续耦合实验运行持续时间长达 192 h。运行过程中，细胞浓度维持在 0.84~4.00 g/L，发酵液中ABE的总浓度为5.14~17.54 g/L，葡萄糖浓度大约为16.08~35.15 g/L，总体积产率为0.36 g/(L?h)。实验结果表明，膜生物反应器系统运行稳定，发酵-渗透汽化分离连续耦合生产ABE的操作模式具有可行性和优越性。

Abstract:

Acetone-Butanol-Ethanol (ABE) fermentation continuously coupling with pervaporation was conducted in a continuous and closed-circulating fermentation system by combining clostridium acetobutylicum and PDMS membrane bioreactor. The continuous fermentation was successfully lasted for 192 h, and the corresponding kinetic behaviors were studied. During the continuous fermentation, cell concentrations of 0.84~4.00 g/L, glucose concentrations of 16.08~35.15 g/L, the total solvents concentrations of 5.14~17.54 g/L in the fermentation broth and ABE productivity of 0.36 g/(L?h) were achieved. The results showed that ABE fermentation continuously coupling with pervaporation in a PDMS membrane bioreactor system is feasible and promising.