低分子量硫酸软骨素的制备及其性质研究

Preparation and properties of low molecular weight chondroitin sulfate

摘要:

本文以过氧化氢 铜离子（Ⅱ）体系自由基法降解制备得到低分子量硫酸软骨素，并采用化学组成与元素分析、红外光谱、原子力显微镜等物理化学方法进行结构表征和物化性质分析。研究结果表明，铜离子催化氧化降解后硫酸软骨素重均分子量从56596Da降到6906Da，己糖醛酸、氨基己糖及硫酸根等主要成分变化不大，差异不显著，降解前后热分解特性一致。原子力显微镜分析发现，降解前后硫酸软骨素在水中均以多股螺旋棒状形式存在，降解过程中，螺旋链部分断裂。结合红外光图谱，可以推测自由基引起的降解主要是造成硫酸软骨素二糖间β-1,4糖苷键断裂，而对β-1,3糖苷键无影响。

Abstract:

Low molecular weight chondroitin sulfate was first prepared by free radical polymerization with hydrogen peroxide in the presence of copper(Ⅱ) iron. Its characterization and physicochemical properties were investigated by physicochemical methods including chemical composition, elementary analysis, IR and AFM. The result showed that molecular weight of chondroitin sulfate from 56956Da to 6906Da by copper catalytic oxidation. The major ingredient such as hexuronic acid, hexosamine and sulfate radical were no significant difference after degradation. The TG-DSC analysis showed the mechanism of thermal decomposition is similar. The AFM analysis indicated this samples exists in aqueous solution with stranded spiral. Part spiral is breaking during degradation. IR analysis showed that the degradation process may caused β-1,4 glycosidic bond fracture but no effect on β-1,3 glycosidic bond.