化学酶法制备D-丝氨酸

Chemo-enzymatic synthesis of D-serine

摘要:

本文以DL-丝氨酸为原料，先采用氨基酸关环法化学合成酶的作用底物——DL-5-羟甲基海因，收率93.5%。然后该底物在酶的作用下，起始pH 9.0，培养温度38℃，转化时间42 h，经过一系列的酶解反应即得到D-丝氨酸，收率为81.7%。

Abstract:

The substrate DL-5-hydroxymethylhydation was synthesized in a chemical process from DL-serine by amino acid cyclization method with the yield of 93.5%. And then D-serine was produced by the biotransformation of DL-5-hydroxymethylhydation under the condition of pH 9.0,temperature of 38℃ and the conversion time for 42 hours with the yield of 81.7%.