单分散高分子微球的制备及色谱性能考察

Preparation and Chromatographic Characterization of Monodisperse Polymeric Microspheres

摘要:

以二乙烯基苯（DVB-55）和二乙二醇二甲基丙烯酸酯(DGD)为共聚单体，采用沉淀聚合法在乙腈溶剂中一步制得了聚（二乙烯基苯-co-二乙二醇二甲基丙烯酸酯）微球[P(DVB-DGD)]。用FT-IR、SEM和BET等方法对P(DVB-DGD)进行表征，结果表明两种单体在聚合过程中实现了共聚，数均粒径为3.6 μm的单分散微球表面未见大孔结构，微球比表面积为2.9 m2/g，平均孔径为9.1 nm。以两组试样为探针分子，采用反相液相色谱模式考察了P(DVB-DGD)填充的新型固定相的色谱性能，结果显示组分获得了尖锐对称的峰形。色谱柱呈现了较高柱效和良好色谱性能，表明沉淀聚合在HPLC固定相方面具有较好应用前景。

Abstract:

Poly(divinylbenzene-co-diethyene glycol dimethacrylate) [P(DVB-DGD)] microspheres were prepared by precipitation polymerization in acetonitrile solvent with divinylbenzene-55 and diethyene glycol dimethacrylate as comonomers. FTIR、SEM and BET were applied to characterize P(DVB-DGD). FT-IR showed that two monomers copolymerized during the precipitation polymerization process. SEM indicated that no macroporous structure could be observed and the average number diameter of the monodisperese microspheres was 3.6 μm. The surface area and the average diameter of P(DVB-DGD) were 2.9 m2/g and 9.1 nm, respectively. With two samples as probes, the chromatographic performance of novel stationary phase packed with P(DVB-DGD) was evaluated by reversed-phase liquid chromatographic mode. The chromatographic results showed that the peak shape of the analytes was sharp and symmetry. The column exhibited high column efficiency and excellent chromatographic performance，which indicated that precipitation polymerization showed great potency of stationary phase used in HPLC.