微生物Mucor sp.转化肉桂酸生成苯乙酮降解途径分析

Analysis of Degradation Pathways from Cinnamic Acid to Acetophenone by Mucor sp.

摘要:

利用GC-MS检测Mucor sp.转化肉桂酸的产物，对微生物Mucor sp.降解肉桂酸的途径进行了分析。β-苯乙醇首次作为微生物Mucor sp.转化肉桂酸生成苯乙酮的中间体在产物中被检测到。Mucor sp.降解肉桂酸的途径是:在肉桂酸的侧链双键上先发生环氧化反应得到3-苯基-2,3-环氧丙酸，环氧化合物经脱羧、还原开环得到中间体β-苯乙醇，β-苯乙醇经羟基转移和进一步催化氧化生成苯乙酮。

Abstract:

The metabolic pathway for the degradation of cinnamic acid with Mucor sp. was studied by analyzing metabolites using GC-MS. β-phenethyl alcohol, for the first time, was detected as an intermediate in transforming cinnamic acid to acetophenone by Mucor sp. The metabolic pathway of cinnamic acid was supposed: epoxidation reaction was occurred on the double bond of cinnamic acid side chain to generate ethyl 2,3-epoxy-3-phenylpropionic acid, the epoxide was converted to β-phenethyl alcohol via decarboxylation and ring-opening reaction, lastly, acetophenone was produced from β-phenethyl alcohol via hydroxyl migration and oxidation reaction.