大孔吸附树脂纯化黄花蒿黄酮及其抗氧化活性

Study on Purification and Antioxidant Activity of Flavonoids from Artemisia Annua L.by Macroporous Resins

摘要:

摘要：本文对大孔树脂纯化黄花蒿黄酮的工艺条件进行了研究与优化，并对纯化后的黄酮进行了抗氧化活性的研究。比较了AB-8，DM-101，DA-201，D-101及SD-401对黄花蒿中黄酮类物质的吸附及解吸附性能，结果显示D-101的综合效果最佳。通过D-101大孔吸附树脂动态吸附解吸实验，获得较佳的纯化工艺：上样液黄酮浓度为1.55 mg/mL，流速以2 mL/min，上样100 mL后，用蒸馏水洗脱至溶液无色，再用250 mL的70%乙醇以1 mL/min洗脱。在此条件下，黄酮的含量从20.10%提高到80.32%，洗脱率达到91.08%，黄酮回收率为68.25%。黄花蒿黄酮对油脂有明显的抗氧化性作用；黄花蒿黄酮对植物油的抗氧化能力强于柠檬酸和抗坏血酸，对动物油脂的抗氧化能力稍弱于抗坏血酸而略强于柠檬酸。

Abstract:

Abstract: The purification of flavonoids from Artemisia Annua L.was studied by macroporous adsorption resins and antioxidant activity of the flavonoidswas was investigated. The adsorption and desorption effects of AB-8, DM-101, DA-201, D-101, SD-401 on flavonoids from Artemisia Annua L. were compared. Results show that the D-101 macroporous resin has the best adsorption and desorption effect. Through the investigation on the dynamic parameters of adsorption and desorption of D-101macroporous resin, the optimal conditions was obtained, adsorption conditions: the Sample concentration of flavonoids 1.55 mg/mL, flow velocity 2 mL/min, Sample volume 100 mL, then distilled water to wash, desorption conditions: eluant concentration 70% ethanol, eluant volume 250 mL, flow velocity of eluant 1 mL/min. Under the optimal conditions, the content of flavonoids is raised from 20.10 % to 80.32 %, ratio of desorption reaches 91.08%, and the yield of total flavones is 68.25%. The flavonoids from Artemisia Annua L. reveale obvious antioxidant effect on oil and fat. The antioxidant activity of flavonoids from Artemisia Annua L. is superior to not only ascorbic acid but also citric acid on oil, and it is somewhat higher than that of citric acid but inferior to ascorbic acid on fat.