蒜头果木质素提取及成分分析

Analysis of Chemical Component of Lignin Extract from Malania oleifera

摘要:

采用一般碱法、超声波辅助和微波辅助从蒜头果壳和枝中提取木质素，分别测定了蒜头果壳和枝中木质素含量及木质素的提取率，并对所提取木质素进行红外和紫外光谱分析。克拉森木素定量法测得蒜头果壳和枝中木质素含量别为44.41(±0.65)%和34.57(±0.52)%，在碱浓度0.5mol/L、碱液量30mL/g、超声波处理时间1h、水浴温度40℃提取工艺条件下，蒜头果壳和枝的木质素提取率分别为45.21(±0.59)%和63.78(±0.73)%。紫外和红外图谱显示超声波辅助方法提取的木质素保持了木质素原有结构，存在明显的愈疮木基和紫丁香基苯环结构。并通过粘度法比较了三种不同提取方法所得到的木质素相对分子质量。

Abstract:

Lignins were extracted from Malania oleifera shell and branch by traditional alkaline method, ultrasound-assisted method and microwave-assisted method．The yields and the contents of lignins in Malania oleifera shell and branch were determined. Meanwhile, the lignins obtained were structurally analyzed by infrared and (IR) and UV spectroscopy. The contents of lignin in Malania oleifera shell and branch were 44.41(±0.65)% and 34.57(±0.52)% respectively by Klason method, the lignins extraction rates reached 45.21(±0.59)% and 63.78(±0.73)% respectively from Malania oleifera shell and branch under the conditions of ultrasound- assisted extraction at 40℃ water bath for 1 hour with 0.5 mol/L KOH water as the solvent and the solvent/material ratio of 30:1 (mL/g). UV and IR spectral analyses showed that the lignin extracted by ultrasound-assisted method remained its original structure，there were many syringyl and guaiacyl groups in Malania oleifera shell and branch. And compared the relative molecular weight of lignins extracted by three different extraction methods with viscosity method.