蕨提取液对亚硝酸根清除的工艺

Extracts to nitrite elimination from Pteridium aquilinum var. latiusculum

摘要:

利用分光光度法,研究蕨老茎叶提取液体外清除亚硝酸盐的能力. 在单因素试验基础上，利用Design-Expert Software8.06设计了响应曲面试验，考察乙醇体积分数、提取时间、料液比以及提取温度四因素对蕨老茎叶提取液清除亚硝酸盐效果的影响。蕨老茎叶中清除亚硝酸盐有效成分的最佳提取工艺条件为：液固比（V:W）为69：1，用体积分数71%的乙醇在81℃下水浴回流90min，此时提取液对亚硝酸盐的最大清除率为70.21%。模型预测对亚硝酸钠的清除率为69.50%.

Abstract:

The nitrite scavenging capacity of extracts from Pteridium aquilinum var. latiusculum was evaluated in vitro by spectrophotometric method. On the basis of single factor tests, response surface analysis was designed by Design-Expert Software 8.06. The effects of extraction time, ethanol concentration, ratio of liquid to solid, and temperature on the nitrite elimination were investigated. The results showed that the optimal extraction conditions were as follows: with 71% ethanol as the extraction solvent and a ratio of liquid to solid of 69 ml/g, extraction at 81℃ for 90 min. The predicted of elimination of sodium nitrite was 69.50%, close to the practical elimination of sodium nitrite was 70.21%.