利用制革废弃猪毛提取角蛋白

PREPARATION OF KERATIN BASED ON PIGHAIR OBTAINED FROM TANNERY

摘要:

动物毛发经还原法降解所得角蛋白，不仅可以保持其二级结构，还带有大量巯基，可用于制备不同结构和性能的蛋白质-聚合物混杂体(Protein-polymer hybrids, PPHs)生物材料。本论文采用尿素/焦亚硫酸钠还原体系降解制革废弃猪毛制备角蛋白，优化的降解条件为：pH 6.5，焦亚硫酸钠浓度1.0 mol/L、尿素浓度14 mol/L、十二烷基硫酸钠浓度0.2 mol/L，时间8h，降解率达55%-60%。利用红外光谱（FT-IR）和热重分析（TGA）以及聚丙烯酰胺凝胶电泳(SDS-PAGE)对降解产物进行分析，表明其具有与原猪毛类似的结构特征和热性能，二级结构得以保持，相对分子质量分布于13kDa-15kDa、45kDa-50kDa 和 52kDa-55kDa三个谱带。巯基含量分析结果表明，角蛋白中约38.9%的硫原子以自由巯基的形式存在，含量丰富的巯基适于作为化学改性反应活性点。

Abstract:

Due to its sustained secondary structure and plenty of thiols, Keratin obtained via reduction system could be used to prepare Protein-polymer hybrids(PPHs) bio-materials. In the paper, pig hair was dissolved in reduction mixture under optimized conditions (pH 6.5, NaS2O5 1.0 mol/L, Urea 14 mol/L, SDS 0.2mol/L, 8h, 65℃) to extract keratin, it gave conversion of 55-60%. The FT-IR spectra and TGA curves of result keratin, when compared with the original hair, showed that its secondary structure was maintained. Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis indicated that the keratin had molecular weight distribution of three bands (13kDa-15kDa、45kDa-50kDa and 52kDa-55kDa). The result of Ellman test indicated that free thiols of keratin covered about 38.9% of total sulfur in keratin.