罗非鱼鳞胶原蛋白肽铁螯合物制备工艺的优化

Optimization of preparation process for iron chelate of tilapia scale collagen peptide

摘要:

根据响应面试验设计原理，设计四因素三水平的响应面试验进行优化罗非鱼鳞胶原蛋白肽铁螯合物的制备工艺，建立了肽铁螯合率与pH、肽铁质量比、时间、温度的数学模型。结果表明，pH、肽铁质量比、时间对肽铁螯合率影响显著。最佳制备工艺参数为：pH 5.4、温度40℃、肽铁质量比3:1、时间41 min。在此条件下得到肽铁螯合率的验证值为90.49%，与预测值无显著性差异。

Abstract:

According to the experimental design principle of response surface methodology, four factors and three levels of response surface experiment was designed to optimize the preparation process of the tilapia scales collagen peptide iron chelate. A mathematical model was established to describe peptide iron chelate rate as a function of pH, peptide iron mass ratio, time and temperature. The results show that pH, peptide iron mass ratio and time have a significantly effect on the peptide iron chelate rate. The optimal preparation conditions is pH 5.4, temperature 40 ℃, the peptide iron mass ratio 3:1, and time 41 min. Under the optimal preparation process, the practical value of peptide iron chelate rate is 90.49%, which has no significant difference with the predicted value.