含α-O-4和β-5结构木质素模型物的酶促合成与表征

Enzymatic synthesis and characterization of lignin model compound composed of α-O-4 and β-5 linkages

摘要:

在辣根过氧化物酶/H2O2的催化体系下，以异丁香酚为原料，酶促合成包含α-O-4和β-5的木质素模形物。研究了不同条件下对合成的影响, 并用红外分析仪（IR）、元素分析仪(EA)、气质色谱联用（GC-MS）、核磁共振仪(NMR)对产物的结构进行表征。结果表明，异丁香酚（5mmole）和双氧水的摩尔配比为1:1，反应温度为50℃, 辣根过氧化物酶的用量为1.5ml（10g/L）,反应体系为丙酮与磷酸氢二钠和磷酸二氢钠组成（pH=5.83）的混合体系35ml，体积比为3:4,反应时间为2h时，合成反应产率可以达到90%以上。

Abstract:

A lignin model compound composed of α-O-4 and β-5 linkages was synthesized by the radical polymerization with isoeugenol as the raw material and horseradish peroxidase (HRP)/H2O2 as the catalyst. The process variables of the synthesis were investig- ated and the structure of the product was characterized by IR, GC-MS, EA and NMR. It is found that a more than 90% of yield was achieved under the optimal reaction conditions of 1:1 molar ratio of isoeugenol to H2O2, 1.5ml of 10g/L HRP, 3:4 of volume ratio of acetone to phosphate buffer solution, 50℃ of the reaction temperature and 2h reaction time.