自溶-超声波耦合法提取啤酒废酵母中β-(1,3)-D-葡聚糖研究

Autolysis of Ultrasonic Coupling Extraction from Waste Beer Yeast β- (1, 3) - D - Glucan Research

摘要:

采用自溶-超声波耦合法提取啤酒废酵母中β-(1,3)-D-葡聚糖。首先以自溶率为考察指标，将单因素和正交试验相结合，结果显示pH5.0条件下，5% NaCl和1.5 %乙酸乙酯混合添加可以最大程度的促进酵母细胞的自溶，从而促进酵母细胞质壁分离。进而采用超声波法从自溶后收集的细胞壁沉淀中提取β-(1,3)-D-葡聚糖，以2% NaOH作超声溶剂，利用Box-Behnken设计考察了超声功率，超声时间和超声次数对葡聚糖提取率的影响，确定最佳提取条件为：超声功率550W，超声时间33min，超声次数2次，在此条件下葡聚糖提取率达到50.5%，与纯超声波提取葡聚糖法相比提取率提高了2倍。

Abstract:

Abstract: Autolysis-ultrasonic coupling method was adopted to extract β- (1, 3) -D- glucan from waster beer yeast. Firstly, single factor and orthogonal test were designed to study the effect of different autolysis promoters on yeast autolysis. The results showed that the mixture of 5% NaCl+1.5% ethyl acetate can effectively promote yeast autolysis. Furtherly, ultrasonic method was used to extract β-(1, 3)-D-glucan from the cell wall deposition harvested after autolysis. The effects of ultrasonic power, ultrasonic time and ultrasonic times were explored in 2% NaOH solvent by response surface methodology. The results indicated that the optimum parameters were ultrasonic power 550 W, ultrasonic time 33 minutes, 2 ultrasonic times. Under the above conditions, the extraction rate of β-1, 3-glucan was up to 50.5 %, which was doubled as compared with pure ultrasonic method without autolysis pretreatment.