5种小檗科植物多糖含量及抗氧化活性分析

Contents and Antioxidant Activities of Polysaccharides in 5 Species in the Family Berberidaceae

摘要:

应用苯酚-硫酸比色法测定了南天竹(Nandina domestica)、日本绿叶小檗(Berberis thunbergii)、红叶小檗(Berberis thunbergii var. atropurpurea)、十大功劳(Mahonia fortunei)和阔叶十大功劳(Mahonia bealei)的多糖含量，应用ABTS[2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐]和DPPH(1,1-二苯基-2-三硝基苯肼)方法分析了5种小檗科植物多糖的抗氧化活性。结果表明，南天竹、日本绿叶小檗、红叶小檗、十大功劳和阔叶十大功劳的多糖含量平均值分别为27.82%、14.52%、12.14%、14.44%和16.80%，清除ABTS•+自由基的EC50值分别为0.1688、0.5436、0.1604、0.3991和0.1689mg/mL，清除DPPH自由基的EC50值分别为0.0678、0.3769、0.2104、0.2917和0.2224mg/mL。相同属植物多糖的含量无显著差异，不同种植物多糖的含量及抗氧化活性差异较大。多糖样品的抗氧化活性存在明显的剂量依赖性，基于量效关系建立的数学模型具有较高的拟合度。南天竹、阔叶十大功劳多糖含量较高且抗氧化活性较强，具有一定的开发利用价值。

Abstract:

Contents of polysaccharides in five species in the family Berberidaceae, including Nandina domestica, Berberis thunbergii, Berberis thunbergii var. atropurpurea, Mahonia fortunei and Mahonia bealei were determined by phenol-sulfuric acid method, and antioxidant activities of polysaccharides in these five species were evaluated by ABTS (2,2’-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) diammonium salt) and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays. Average contents of polysaccharides in Nandina domestica, Berberis thunbergii, Berberis thunbergii var. atropurpurea, Mahonia fortunei and Mahonia bealei were 27.82%, 14.52%, 12.14%, 14.44% and 16.80%, respectively. EC50 values of ABTS•+ radical-scavenging capacity were 0.1688, 0.5436, 0.1604, 0.3991 and 0.1689 mg/mL, respectively, and EC50 values of DPPH radical-scavenging capacity were 0.0678, 0.3769, 0.2104, 0.2917 and 0.2224 mg/mL, respectively. There is no significant difference of polysaccharide contents among the same genera, while polysaccharide contents and antioxidant activities are different among various species. Both ABTS•+ and DPPH radical-scavenging capacities were dose dependent, and mathematical model based on dose-effect relationship had high goodness of fit. Contents and antioxidant activities of polysaccharides in Nandina domestica and Mahonia bealei were relatively high, which showed great potential for exploitation and utilization.