β-伴大豆球蛋白纳米粒的自组装及Vc释放行为

Self-assembly and Release Behavior of Vc-loaded β-conglycinin Nanoparticles

摘要:

采用去溶剂自组装制备了β-伴大豆球蛋白纳米粒。利用浊度法研究了蛋白质量浓度、乙醇与蛋白溶液体积比、戊二醛用量对纳米粒径的影响，并测定包埋在纳米粒中Vc的释放行为。结果表明，蛋白质量浓度为10-12 g/L、乙醇与蛋白溶液体积比为4:1、戊二醛(1.5 g/L)用量为0.5 mL 时，制备的纳米粒分散性较好，纳米粒径主要分布在350-380 nm。交联纳米粒的Vc释放平稳，未交联纳米粒的Vc释放速率出现突然加速现象；Vc在pH3.0、7.4缓冲溶液中的释放机理分别为Fickian扩散和反常输运。

Abstract:

β-conglycinin nanoparticles were prepared by self-assembly with desolvation method. The effect of protein concentration, volume ratio of ethanol to protein solution and amount of glutaraldehyde on nano-size was studied using turbidity method, and the release behavior of Vc embedded in the nanoparticles was determined. The results showed that the nanoparticles had better dispersity when the protein concentration, the volume ratio of ethanol to protein solution and the amount of glutaraldehyde (1.5 g/L) was 10-12 g/L, 4:1 and 0.5 mL, respectively. The nano-size was mainly around 350-380 nm. Vc in crosslinked nanoparticles released smoothly while Vc in uncrosslinked nanoparticles had a sudden accelerated phenomenon in the release rate, and the release mechanism of Vc in pH3.0 and 7.4 buffer solutions was Fickian diffusion and anomalous transport, respectively.