盐酸或氨基磺酸催化水解斯替夫苷制备甜菊醇

Steviol preparation catalyzed by hydrochloric acid or sulfamic acid

摘要:

分别以盐酸或氨基磺酸为催化剂水解斯替夫苷(St)，研究底物浓度、反应温度、催化剂用量和反应时间对斯替夫苷水解的影响，着重考察了斯替夫苷的转化率、甜菊醇的产率和异甜菊醇的产率。其中，盐酸催化水解斯替夫苷的最优工艺条件：反应温度为95oC，底物浓度为400mg/ml，盐酸加入量为8% (mol%/St)，反应时间28h；在此优化条件下，根据高效液相分析计算得到斯替夫苷转化率接近100%，甜菊醇产率达83.6%，异甜菊醇产率达12.4%。氨基磺酸催化反应的工艺条件：反应温度为95oC，斯替夫苷浓度为400mg/mL，氨基磺酸加入量为10%(mol%/St)，反应时间31h；在此优化条件下，根据高效液相分析计算得到斯替夫苷转化率接近100%，甜菊醇产率达81.5%，异甜菊醇产率达11.7%。

Abstract:

Experimented the effect of the factors of temperature, St concentration, catalyst amount and reaction time on hydrolysis of stevioside catalyzed by hydrochloric acid or sulfamic acid. The results showed that stevioside conversion was nearly 100% , a yield of steviol reached 83.6% and a yield of isosteviol reached 12.4% catalyzed by hydrochloric acid with the optimum conditions. With the sulfamic acid catalyst, a conversion of stevioside of 100% nearly, a yield of steviol of 81.5% and a yield of isosteviol of 11.7% were obtained with the optimum condition.