核桃青皮中蒽醌类色素提取及其稳定性研究

Extraction and Stability study of anthraquinones pigments from Walnut Green Husks

摘要:

在确定了核桃青皮中蒽醌类色素含量分析方法的基础上，采用L9(34)的正交试验研究了乙醇提取核桃青皮中蒽醌类色素的最佳提取工艺；考察了光照、氧化还原剂、金属离子对核桃青皮提取液中蒽醌类色素稳定性的影响。结果显示：比色法测定核桃青皮中的蒽醌类色素的精密度高，稳定性和重复性好，回收率在101.34%～103.52%之间，线性范围为0.00416～0.0250mg/ml(R=0.9997)；当料液比为1: 30、超声时间为30min、温度为60℃、乙醇浓度为50%时核桃青皮色素的提取率最高。对核桃青皮提取液的色素稳定性研究表明：核桃青皮蒽醌类色素具有较好的耐光性和耐还原性，金属离子K 、Na 、Mg2 、Ca2 、Zn2 对其稳定性影响不大，但Fe3 有明显的增色效果。

Abstract:

On the basis of deterrmined the pigment content analytical method for anthraquinones pigment content, an Orthogonal test L9(34) was used to observed the optimal extraction process of ethanol extract anthraquinones pigments from walnut green husks, and the effects of the pigment stability on oxidants, reducing agents, light, metal ions were also investigated. The results showed that this analysis method has high precision and accuracy (recovery is 90.44% ~ 103.52%), with well stability and good reproducibility. The linearity with correlation coefficient 0.9997 was found within the range of 0.00416 mg/ml to 0.0250 mg/ml. The highest pigment extraction rate can be obtained when solid-liquid ratio is 1:30(g．ml), ultrasonic time is 30min, extraction temperature is 60℃ and ethanol concentration is 50%. Research results for pigment stability also displayed that the metallic ions K 、Na 、Mg2 、Ca2 and Zn2 have no apparent effect, and Fe3 has hyperchromic effect significantly.