氧化葡萄糖酸杆菌的固定化及在鼓泡式反应器中制备乙醇酸

Immobilized of Gluconobacter oxydans Cells for Preparation

摘要:

考察了包埋法固定氧化葡萄糖酸杆菌的过程，并且研究了利用此固定化细胞在鼓泡塔生物反应器中转化乙二醇制备乙醇酸。固定化的最优条件为：聚乙烯醇的最佳质量分数为8%，海藻酸钠为0.8%，最适湿细胞包埋量为每毫升凝胶0.27g。固定化细胞对酸碱的抗性和热稳定性均较游离细胞有所提高。利用固定化细胞在鼓泡式反应器中转化乙二醇制备乙醇酸，在乙二醇浓度70g/L的条件下，首批转化率达到92.6%，并且可以实现连续转化6个批次，平均转化率在85%以上。

Abstract:

Immobilization of Gluconobacter oxydans cells and conversion of ethylene glycol to glycolic acid in bubbling reactor were investigated. The optimum of immobilization was 8%PVA, 0.8% sodium alginate and 0.27g cells biomass per milliliter gel. The immobilized cells were more stable than free cells in pH and thermal stability. Conversion of ethylene glycol to glycolic acid was carried out in bubbling reactor. When the initial ethylene glycol was 70g/L, the first cycle conversion was 92.6%, and it could successively convert six cycles, the average conversion was about 85%.