聚多巴胺功能化纳米银粒子制备及抗菌性能研究

Study on Preparation and Antibacterial Properties of Polydopamine Functionalized Ag Nanoparticles

摘要:

采用一步法，以聚多巴胺 (PDA) 为还原剂和保护剂，制备PDA功能化的纳米银粒子 (PDA-nanoAg)。提出PDA-nanoAg合成机理，并考察其在水相中的分散稳定性。通过紫外-可见吸收分光光度计 (UV-Vis)、透射电子显微镜 (TEM)、傅里叶变换红外光谱仪 (FT-IR) 对产物形貌和结构进行表征。采用肉汤稀释法测试PDA-nanoAg的抗菌性能。结果表明，所制备的PDA-nanoAg平均粒径为50 nm，具有良好的稳定分散性；对埃希氏大肠杆菌 (E. coli)、金黄色葡萄球菌 (S. aureus) 的最小抑菌浓度 (MIC) 为7.56 mg/L，最小杀菌浓度 (MBC) 为30.24 mg/L。

Abstract:

Polydopamine (PDA) functionalized silver nanoparticles (PDA-nanoAg) were synthesized by one-step method with PDA as the reductant and protectant. The mechanism for preparation of PDA-nanoAg was proposed. The stable dispersion duration of the as-synthesized Ag nanoparticles in water was also observed. The structure and morphology of PDA-nanoAg particles were investigated by ultraviolet-visible (UV-Vis) absorption spectroscopy, transmission electron microscopy (TEM), and Fourier-transform infrared (FTIR) spectrometry. The antibacterial activity of PDA-nanoAg nanoparticles against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were investigated by broth dilution. The results indicated that the average diameter of the Ag nanoparticles was 50 nm, and PDA-nanoAg particles exhibited good stability in water over a long period of time. The minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of the as-synthesized Ag nanoparticles against E. coli and S. aureus were 7.56 mg/L and 30.24 mg/L, respectively.