离子液体/盐双水相萃取分离锁阳中熊果酸

Separation ursolic acid in Cynomorium songaricum Rupr by

摘要:

利用1-丁基-3-甲基咪唑氯([Bmim]Cl)和磷酸氢二钾(KH2PO4)双水相体系萃取分离锁阳中熊果酸，首先对[Bmim]Cl、KH2PO4、熊果酸粗提取液质量分数、NaCl质量分数、pH进行单因素研究，确定双水相体系组成为25%[Bmim]Cl-21%KH2PO4。然后利用Box-Benhnken Design实验，对黄酮粗提取液质量分数、pH、NaCl质量分数进行了优化，结果表明：粗提液质量分数8.5%、NaCl质量分数1%、pH 4.7，熊果酸萃取率可达97.85%。与预测值98.3%接近。说明本优化工艺具有可行性。

Abstract:

In this study the potential use of ionic liquid-based aqueous two phase system (ILATPS) for the extraction of ursolic acid from Cynomorium songaricum Rupr was evaluated and optimized. The effects of independent variables(the mass fraction of [Bmim]Cl and KH2PO4, mass fraction of crude extract, mass fraction of NaCl and pH)were evaluated by single factor analysis. The optimization ATPS of 25%[Bmim]Cl-21%KH2PO4were selected based on their higher upper phase partitioning coefficient and the recovery .Using the optimized ILATPS solvent, the Box-Benhnken Design results showed mass crude extract 8.5%,NaCl 1%, pH4.7 were the optimal ILATPS extraction conditions. Maximum yield of extracted ursolic acid from the experiments was determined to be 97.85% under the optimal conditions, which was in good agreement with the predicted one of 98.3%. In conclusion, the optimized process has good reliability.