天然固定化木瓜脂肪酶催化芳香仲醇动力学拆分的研究

Kinetic Resolution of Racemic Aromatic Secondary Alcohols Catalyzed by a Naturally Immobilized Carica papaya Lipase

摘要:

将木瓜来源的廉价天然固定化脂肪酶CPL用于手性拆分芳香仲醇，以4-苯基-2丁醇作为模式底物，考察了反应温度、反应溶剂、酰基供体以及底物摩尔比等关键因素对CPL拆分能力的影响，结果表明：适宜的反应温度30~45℃，酰基供体为活性乙烯酯，正己烷或环己烷作为反应溶剂，底物/酰基供体摩尔比1:2，在此条件下底物的转化率接近50%，e.es大于99%，反应的对映体比值E大于200。CPL对其它结构类似的芳香仲醇也具有很好的拆分能力。本研究为手性芳香仲醇的酶催化制备提供了新的催化剂选择。

Abstract:

The low cost naturally immobilized lipase CPL from the Carica papaya was used to catalyze kinetic resolution of aromatic secondary alcohols. Taking the 4-phenyl-2-butanol as the model substrate, the effects of reaction temperature, reaction solvent, acyl donor and substrate molar ratio on the resolution ability of CPL were investigated. The results showed that the optimum reaction conditions were as follows: reaction temperature of 30~45℃, acyl donor of active vinyl ester, n-hexane or cyclohexane as reaction solvent and substrate/acyl donor molar ratio of 1:2. Under these conditions, the substrate conversion was close to 50%, e.es was more than 99%, and enantiomeric ratio E was higher than 200. CPL also showed good resolution ability to other similar structure aromatic secondary alcohols. This work provides a new biocatalyst alternative for economic preparation of chiral aromatic secondary alcohols.